Some have been complaining of my absence in the blogging world. *cough heather* You want an update? OH.. I will give you an update…
Here is what has been tying me up lately: I have a real job. We are not talking like the average summer job. I work 8(ish)-5(ish) EVERY day like a real adult! On the first real day (after orientation day) I arrived excitedly at 7:30 AM just to be safe- because I really wasn’t sure how long I would be lost before finding the physiology dept. (Although I had orientation the day before, they failed to actually orient me in terms of direction) I held my head high with my id badge proudly clipped onto my shirt, just waiting for the day ahead. I parked, climbed out of my car, and then began the trek to the actual building (approximately 12.3 miles away from aforementioned parking spot). I traversed vast empty plains of parking spots reserved for the important people who get the close lots, and then made my way through endless halls that all look the same. I got to the physiology department at 7:45, and found…nobody.
This was why there were vast empty plains of parking spots, apparently.
The secretaries wandered in a little after 8, and so I waited. I found a comfy chair located in the hallway and sat contentedly. I had many papers published in journals by Dr. Toney, which he had given me the day before as "some light reading" for "background info". Light reading my butt. So I read until 8:45 when Dr. Toney strolled in. Turns out that researchers keep a later schedule than most.
Anyway, UT Health Science Center in San Antonio is my kingdom, and Dr. Toney's autonomic neurophysiology lab my playground. We are researching several aspects of the paraventricular nucleus and its role in cardiovascular control- among many other things. Oh, and when I say "we" I mean "they". I can't even find my way to the bathroom, much less implant flourescent microbeads in the rostral ventrolateral medulla of rats.
Dr. Toney has a project that I will "spearhead" (which could be a synonym for "ruin miserably"), but I can't start until I get some rats of my very own. So for now I remain the useless summer student/intern/peon/slave. I guess if I expect them to think of me as any more than "the undergraduate", I should first learn how to say the word
immunoglobulin without stuttering. A pang of fear seizes me every time I know it is coming up in conversation, because no matter how hard I try it usually comes out sounding something like "emooonugoblilun". It reminds me of 6-yr-olds that consistently say
pusketti instead of
spaghetti, or of 21-yr-olds named David Pyle who consistently say
ambleance instead of
ambulance.
Needless to say, this difficulty kind of embarrasing.
I work with some interesting folks, so here is the lineup:
Dr. Glenn Toney - P.I. This is my boss, and since he only researches and doesn't teach, he is offically a "P.I." It took me a while to realize that PI meant
primary investigator and not
private investigator. It is too bad, because I had all these fanciful dreams going in black and white. I was just waiting to walk dramatically in his office with a tear on my cheek- and see him sitting smugly at his desk, feet up, gum on the bottom of his shoe, and a hat cocked to one side...
(In a desperate, raspy voice, almost a whisper)
"Toney, I need your help""Ah, you again…well you've come to the right place, darlin’. Tell me what you need"
"It’s just that..no, no I ..I can’t" (turns head away and covers mouth)
"It’s not…?!?"
(Sobs)
"It is.. it is….."(With disgust) "that rat!"
Anyway, once I found out he was merely a primary investigator and not a private one, I decided not to disclose any dramatic secrets in hopes of retribution and safety. Ok, so I don’t have any dramatic secrets-especially none that would require protection. In reality, we really do talk about rats though. Our conversations are usually him talking, me nodding. Literally this is how one of our conversations went the other day when he called the lab from his office…
"’Hey there Kara, it’s Glenn here"
"Oh hey" (I feign peppiness as fear wells up inside as I imagine him quizzing me on the ins and outs of neuroscience. This always happens, even though he has never come close to quizzing me.)
"So I have been reading the literature, and it looks like the hypoxyprobe kit has a primary antibody conjugated to FITC, and then the secondary is anti-FITC and labeled with HRP"
"OH… ok""So this replaces the avidin-biotinylated system we talked about and serves as the amplification step"
"uh huh…ok""I am not positive...but maybe since it is FITC labeled we will need to incubate in the dark to prevent photobleaching, but I really doubt the binding of the secondary antibody will depend on the fluorescence of the primary FITC because I can’t imagine they would make a system that is thrown off by such a small part of the molecule being changed"
"yeah.""Now I am going to ramble on with lots of scientific things that you don’t understand, but in such a blasé manner as to indicate that either you really should know them or I am so far removed from the real world that I forget these are not normal things to know."
"I am gonna keep on nodding and saying such radically profound things as yeah, and ok, pretending I comprehend every word and concept in hopes that it really isn’t important to know for my study, and if it is, maybe I can look it up on the internet later."So that is usually how it goes.
AlfredAlfred is the lab tech that I shamelessly follow around. I am like a little puppy. It probably gets annoying, but he is sort of the one in charge of me, and so I watch all his surgeries and help him take care of rats and such. He also is a very sweet guy, so he has no problem allowing me to go almost everywhere with him.
Sometimes I think that maybe he finds some really simple task for me to do just so he can have a little time to himself, kind like when a person throws the tennis ball far into the woods to keep the puppy occupied for a while. I promise I have gotten better, and don’t totally follow him around all the time anymore.
Andrei-the silent RussianAndrei doesn’t speak. The only sound I have heard from him is the slight grunt he gave to accompany the head nod when we were introduced. Well, that is all I have heard out of him in English, anyway…assuming it was an English grunt and not a Russian one. Apparently the world of research attracts lots of Russians, because the physiology department has plenty, and they all congregate at Andrei’s desk and speak Russian together.
I have never actually seen the man do any work. He just sits and talks to other Russians…in Russian. Whenever I am near his desk I feel like I am on a Hunt for Red October. What if they are planning something? They could easily be conspirators, and the physiology department would never see it coming.
I heard on the down low from Alfred that there is some drama and bitterness with Andrei, because he thought he was getting a faculty position but instead they put him as a post doc in Toney’s lab. It is not talked about, and I was told I absolutely must never refer to Andrei as a post doc- which is fine, because even if I were to speak to Andrei, (equivalent of speaking to a wall- a foreign wall), I would never say "what’s up, post doc!". That would require a carrot in my hand, large ears, and a name like bugs.
*Please in no way think I have anything against Russians. I am all about glasnost.
Sean "I am way proud of myself" StockerSean is the post doc, and is very quick to tell you that he actually has a faculty position at University of Kentucky, and they are in the process of building him his own lab, and oh yeah, if he can find a way to slip it in… they are investing a whole lot of money in him. Cool, Sean. Good for you.
In all fairness the guy does have a reason to be cocky. He is really intelligent, and has accomplished quite a bit. It isn’t easy to get a faculty position- and I really am happy for him. In fact, he is really nice towards me, and I have every reason to like the guy. I probably would like him a whole lot if he weren’t such a jerk to Alfred. He treats Alfred like dirt, and always has some snide comment that accentuates Alfred’s "lower education" (with only a bachelor’s), and his innate superiority. GET OVER YOURSELF.
He also tends to be something of a drama queen, and just LOVES to diss Dr. Toney behind his back. What is great is that he always tries to soften his insults. " Ya know, Glenn is really one of the smartest guys I have ever met..BUT…" OH shush. We all know that you think
you are the smartest person you have ever met.
Peng( pronounced pong)I really keep wanting to type Pong since that is how it’s said, so I think I will. Pong is a really sweet graduate student. Well, she seems sweet. All I have to judge by is her demeanor and tone when she says hi to me in the morning. We smile at each other too. Our interactions stop there. This is due to something of a language barrier.I really would like to talk to her more though. My dream conversation goes a little something like this:
"Hey Ping!""No, it’s Pong"
"Ping?""Pong"
"Ping.""PONG!"
Just kidding…I would never really do that.
The scruffy little Asian manI don’t really know who he is, but he’s cute. He just kind of wanders into our lab, takes an instrument, and leaves. Sometimes he brings a mouse with him, sits down, and does surgery in one of the rooms in our lab that isn’t used much. I am assuming this is ok since no one ever says anything. Maybe they just let him go because he is a scruffy Asian, and lets face it, those are hard to come by.
(-)H(-)P01This was my first rat, the first one I saw die for the sake of science. To be brutally honest, he died just to teach me how to kill him, but lets not get semantic. With him, I learned how to perfuse a rat.
I actually held his little beating heart between my fingers, and then pierced the left ventricle with a catheter. It was maybe the weirdest and most disturbing thing I have ever had to do.
I just didn’t realize how warm he would be inside. It makes sense- but everything in the lab is so cold and clean- me cutting him open and sticking my fingers in his warm blood was just startling. I later found myself in the role of Lady Macbeth, washing my hands obsessively.
Out damned spot!Anyway, since then I have perfused many rats. When I was recently talking about work with Brian, he excitedly pointed out, " Oh yay, babeh! You are already desensitized to death!" This is true.
The basic idea of perfusion is that after you stick the catheter in the rat’s heart and open the right atrium, you replace all the rat’s blood with PBS first, and then PFA, a fixative. This is to fix and preserve the tissue for later. After he becomes a "ratsicle" as Dr. Toney likes to say, we chop off his head French-Revolution style (yes we have a rat guillotine) and crack off the skull bit by bit to retrieve the brain. Do realize that they are anesthetized /knocked out while we are doing this...and after we cut from the abdominal cavity up through the diaphragm, they can’t wake up because they have no way of breathing. Their heart does still beat, however.
Sorry, that probably grosses most people out.
Anyway, I am forced to give the rats a name for my study, and so as my system goes, he is (-)H(-)P01. (no hypoxia, no probe, #1. My personal study is hypoxia in the brain.) In my heart, however, he was more than that. In death, he has a name, and his name was Ricardo.
Thank you, Ricardo.
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Was that a long enough entry to count as four, Heather?